Journal: BMC Molecular and Cell Biology

Article Title: Sex alters thyroid hormone’s effect on protein O -GlcNAcylation in the aged mouse heart

doi: 10.1186/s12860-025-00543-x

Figure Lengend Snippet: Serum T4 concentrations in aged male (M) and female (F) mice. Euthyroid (CON), hypothyroid (PTU) and hyperthyroid (TH). Data presented with bars for means and one standard deviation. Statistics – one-way ANOVA – correct for multiple comparison- test: Šidák post hoc analysis was performed (n = 3 animals/sex/treatment).

Article Snippet: The mice were fed one of the following diets: 1) euthyroid controls - normal iodine diet (TD.97350, CON), 2) hypothyroid − 0.15% propylthiouracil (PTU) diet (TD.95125), or 3) hyperthyroid (TH) diet (TD.190014) containing 0.58 ppm T3 (#T2877, Sigma-Aldrich, St. Louis, MO) and 2.338 ppm T4 (#T2376, Sigma-Aldrich, St. Louis, MO) purchased from Envigo (Madison, WI).

Techniques: Standard Deviation, Comparison

Journal: BMC Molecular and Cell Biology

Article Title: Sex alters thyroid hormone’s effect on protein O -GlcNAcylation in the aged mouse heart

doi: 10.1186/s12860-025-00543-x

Figure Lengend Snippet: Total heart protein O-GlcNAcylation levels in males (M) and females (F) comparing euthyroid (CON), hypothyroid (PTU) and hyperthyroid (TH). All values are arbitrary units normalized to total protein levels. Data presented with bars for means and standard deviation. Statistics – one-way ANOVA – correct for multiple comparison- test: Šidák post hoc analysis was performed (n = 3 animals/sex/treatment). Image was cropped to obtain the PTU/CON/TH arrangement. Full-length uncropped blots are presented in Supplementary Fig.

Article Snippet: The mice were fed one of the following diets: 1) euthyroid controls - normal iodine diet (TD.97350, CON), 2) hypothyroid − 0.15% propylthiouracil (PTU) diet (TD.95125), or 3) hyperthyroid (TH) diet (TD.190014) containing 0.58 ppm T3 (#T2877, Sigma-Aldrich, St. Louis, MO) and 2.338 ppm T4 (#T2376, Sigma-Aldrich, St. Louis, MO) purchased from Envigo (Madison, WI).

Techniques: Standard Deviation, Comparison

Journal: BMC Molecular and Cell Biology

Article Title: Sex alters thyroid hormone’s effect on protein O -GlcNAcylation in the aged mouse heart

doi: 10.1186/s12860-025-00543-x

Figure Lengend Snippet: Total liver protein O-GlcNAcylation levels in males (M) and females (F) comparing euthyroid (CON), hypothyroid (PTU) and hyperthyroid (TH). All values are arbitrary units normalized to total protein levels. Data presented with bars for means and one standard deviation. Statistics – one-way ANOVA – correct for multiple comparison- test: Šidák post hoc analysis was performed (n = 3 animals/sex/treatment). Full-length uncropped blots are presented in Supplementary Fig.

Article Snippet: The mice were fed one of the following diets: 1) euthyroid controls - normal iodine diet (TD.97350, CON), 2) hypothyroid − 0.15% propylthiouracil (PTU) diet (TD.95125), or 3) hyperthyroid (TH) diet (TD.190014) containing 0.58 ppm T3 (#T2877, Sigma-Aldrich, St. Louis, MO) and 2.338 ppm T4 (#T2376, Sigma-Aldrich, St. Louis, MO) purchased from Envigo (Madison, WI).

Techniques: Standard Deviation, Comparison

Journal: BMC Molecular and Cell Biology

Article Title: Sex alters thyroid hormone’s effect on protein O -GlcNAcylation in the aged mouse heart

doi: 10.1186/s12860-025-00543-x

Figure Lengend Snippet: Heart protein immunoblots for GFAT 1 in males (M) and females (F) comparing euthyroid (CON), hypothyroid (PTU) and hyperthyroid (TH). Immunoblots are shown for GFAT1 protein levels normalized to total protein stain. Data presented with bars for means and one standard deviation. Statistics – one-way ANOVA – correct for multiple comparison- test: Šidák post hoc analysis was performed (n = 3 animals/sex/treatment). The asterisk – unknown band. Full-length uncropped blots are presented in Supplementary Fig.

Article Snippet: The mice were fed one of the following diets: 1) euthyroid controls - normal iodine diet (TD.97350, CON), 2) hypothyroid − 0.15% propylthiouracil (PTU) diet (TD.95125), or 3) hyperthyroid (TH) diet (TD.190014) containing 0.58 ppm T3 (#T2877, Sigma-Aldrich, St. Louis, MO) and 2.338 ppm T4 (#T2376, Sigma-Aldrich, St. Louis, MO) purchased from Envigo (Madison, WI).

Techniques: Western Blot, Staining, Standard Deviation, Comparison

Journal: BMC Molecular and Cell Biology

Article Title: Sex alters thyroid hormone’s effect on protein O -GlcNAcylation in the aged mouse heart

doi: 10.1186/s12860-025-00543-x

Figure Lengend Snippet: Heart protein immunoblots for GFAT2 in males (M) and females (F) comparing euthyroid (CON), hypothyroid (PTU) and hyperthyroid (TH). Immunoblots are shown for GFAT2 protein levels normalized to total protein stain. Data presented with bars for means and one standard deviation. Statistics – one-way ANOVA – correct for multiple comparison- test: Šidák post hoc analysis was performed (n = 3 animals/sex/treatment). Image was cropped to obtain the PTU/CON/TH arrangement. Full-length uncropped blots are presented in Supplementary Fig.

Article Snippet: The mice were fed one of the following diets: 1) euthyroid controls - normal iodine diet (TD.97350, CON), 2) hypothyroid − 0.15% propylthiouracil (PTU) diet (TD.95125), or 3) hyperthyroid (TH) diet (TD.190014) containing 0.58 ppm T3 (#T2877, Sigma-Aldrich, St. Louis, MO) and 2.338 ppm T4 (#T2376, Sigma-Aldrich, St. Louis, MO) purchased from Envigo (Madison, WI).

Techniques: Western Blot, Staining, Standard Deviation, Comparison

Journal: BMC Molecular and Cell Biology

Article Title: Sex alters thyroid hormone’s effect on protein O -GlcNAcylation in the aged mouse heart

doi: 10.1186/s12860-025-00543-x

Figure Lengend Snippet: Heart protein immunoblots for OGT in males (M) and females (F) comparing euthyroid (CON), hypothyroid (PTU) and hyperthyroid (TH). Immunoblots are shown for total protein OGT levels normalized to total protein stain. Data presented with bars for means and one standard deviation. Statistics – one-way ANOVA – correct for multiple comparison- test: Šidák post hoc analysis was performed (n = 3 animals/sex/treatment). Full-length uncropped blots are presented in Supplementary Fig.

Article Snippet: The mice were fed one of the following diets: 1) euthyroid controls - normal iodine diet (TD.97350, CON), 2) hypothyroid − 0.15% propylthiouracil (PTU) diet (TD.95125), or 3) hyperthyroid (TH) diet (TD.190014) containing 0.58 ppm T3 (#T2877, Sigma-Aldrich, St. Louis, MO) and 2.338 ppm T4 (#T2376, Sigma-Aldrich, St. Louis, MO) purchased from Envigo (Madison, WI).

Techniques: Western Blot, Staining, Standard Deviation, Comparison

Journal: BMC Molecular and Cell Biology

Article Title: Sex alters thyroid hormone’s effect on protein O -GlcNAcylation in the aged mouse heart

doi: 10.1186/s12860-025-00543-x

Figure Lengend Snippet: Heart protein immunoblots for OGA in males (M) and females (F) comparing euthyroid (CON), hypothyroid (PTU) and hyperthyroid (TH). Immunoblots are shown for total protein OGA levels normalized to total protein stain Data presented with bars for means and one standard deviation. Statistics – one-way ANOVA – correct for multiple comparison- test: Šidák post hoc analysis was performed (n = 3 animals/sex/treatment). Image was cropped to obtain the PTU/CON/TH arrangement. Full-length uncropped blots are presented in Supplementary Fig.

Article Snippet: The mice were fed one of the following diets: 1) euthyroid controls - normal iodine diet (TD.97350, CON), 2) hypothyroid − 0.15% propylthiouracil (PTU) diet (TD.95125), or 3) hyperthyroid (TH) diet (TD.190014) containing 0.58 ppm T3 (#T2877, Sigma-Aldrich, St. Louis, MO) and 2.338 ppm T4 (#T2376, Sigma-Aldrich, St. Louis, MO) purchased from Envigo (Madison, WI).

Techniques: Western Blot, Staining, Standard Deviation, Comparison

Journal: BMC Molecular and Cell Biology

Article Title: Sex alters thyroid hormone’s effect on protein O -GlcNAcylation in the aged mouse heart

doi: 10.1186/s12860-025-00543-x

Figure Lengend Snippet: Heart protein immunoblots for NAGK in males (M) and females (F) comparing euthyroid (CON), hypothyroid (PTU) and hyperthyroid (TH). Immunoblots are shown for total protein NAGK levels normalized to total protein stain. Data presented with bars for means and one standard deviation. Statistics – one-way ANOVA – correct for multiple comparison- test: Šidák post hoc analysis was performed (n = 3 animals/sex/treatment). Full-length uncropped blots are presented in Supplementary Fig.

Article Snippet: The mice were fed one of the following diets: 1) euthyroid controls - normal iodine diet (TD.97350, CON), 2) hypothyroid − 0.15% propylthiouracil (PTU) diet (TD.95125), or 3) hyperthyroid (TH) diet (TD.190014) containing 0.58 ppm T3 (#T2877, Sigma-Aldrich, St. Louis, MO) and 2.338 ppm T4 (#T2376, Sigma-Aldrich, St. Louis, MO) purchased from Envigo (Madison, WI).

Techniques: Western Blot, Staining, Standard Deviation, Comparison

Journal: BMC Molecular and Cell Biology

Article Title: Sex alters thyroid hormone’s effect on protein O -GlcNAcylation in the aged mouse heart

doi: 10.1186/s12860-025-00543-x

Figure Lengend Snippet: Immunoblots for O-GlcNAc immunoprecipitated ACAA2 in males (M) and females (F) comparing euthyroid (CON), hypothyroid (PTU) and hyperthyroid (TH). (A) Immunoblots show ACAA2 expression in heart tissue immunoprecipitated with the O-GlcNAc RL2 antibody normalized to total ACAA2. Statistics – one-way ANOVA – correct for multiple comparison- test: Šidák post hoc analysis was performed (n = 3 animals/sex/treatment). Asterisks denotes a non-specific band. (B) Immunoblots of O-GlcNAc expression on immunoprecipitated ACAA2 protein. O-GlcNAc bands were normalized to input ACAA2. Statistics – Unpaired t test was performed (n = 2 animals/sex/treatment). Data presented with bars for means and one standard deviation. Full-length uncropped blots are presented in Supplementary Figure 1

Article Snippet: The mice were fed one of the following diets: 1) euthyroid controls - normal iodine diet (TD.97350, CON), 2) hypothyroid − 0.15% propylthiouracil (PTU) diet (TD.95125), or 3) hyperthyroid (TH) diet (TD.190014) containing 0.58 ppm T3 (#T2877, Sigma-Aldrich, St. Louis, MO) and 2.338 ppm T4 (#T2376, Sigma-Aldrich, St. Louis, MO) purchased from Envigo (Madison, WI).

Techniques: Western Blot, Immunoprecipitation, Expressing, Comparison, Standard Deviation